Although all species of the brachypelma genus are protected under cites, few studies have been performed on the genetic structure of the populations of these endangered tarantulas. Download plant biotechnology and molecular markers s. Issr markers usually show high polymorphism kojima et al. The number of alleles ranged from 2 to 8 per primer, with an average of 4. High genetic diversity indices for both marker types 0. Issr markers as a tool to distinguish idt and sss populations. Genetic diversity and genotype identification using issr and rapd markers pdf. These pcr primers bind directly to microsatellites, such as ca n, which are abundant in eukaryotic genomes. Assessment of genetic variation of genus salvia by rapd and issr markers. The mantel test did not show a correlation between genetic distance and the geographical distance of the studied populations. Population genetic structure of the cleistogamous plant species viola pubescensaiton violaceae, as indicated by allozyme and issr molecular markers. Issrs are now being applied to natural populations to address issues such as hybridization. This work aims to find out the phylogenetic relationship among the different okra genotypes using ntsys base upgma analysis of similarity coefficient and detected issr markers linked to. Resolving power is a characteristic of a primer which reflects overall suitability of a marker system for the purpose of identification, as it is related to the number of specimens distinguished by that primer.
Alternatively, previously reported primers can be used. The present study shows that issr markers could generate abundant polymorphism, are reproducible, and are quick for characterization of poplar cultivars. Genetic diversity in barley from west china based on rapd and. File scanned at 300 ppi monochrome, 8bit grayscale, 24bit color using scandall pro 1. Finally, the aflp showed 100 bands, of which 92 were polymorphic 92%. Issr inter simple sequence repeats as genetic markers in.
Issr for intersimple sequence repeat is a general term for a genome region between microsatellite loci. Comparative results of rapd and issr markers for genetic. Pdf inter simple sequence repeat issr polymorphism and. Application of inter simple sequence repeat issr markers. The application of issr markers in genetic variance detection among durian durio zibethinus murr. July genetic homogeneity revealed using scot, issr and rapd markers in micropropagated pittosporum eriocarpum royle an endemic and endangered medicinal plant julie thakur 0 2 3 mayank d. Molecular analysis of chickpea using dna technology has been carried out to identify the diverse genetic base of the cultivars for selected preferential introductions as efficient edible elements. Pdf inter simple sequence repeat issr markers for genetic. Clustering analysis of ethiopian safflower carthamus tinctorius using issr markers baye wodajo1, feysal bushira mustefa2, kassahun tesfaye3 1woldia university, p. It is a simple and quick methodthat combines most of the advantages of microsatellites ssrs andamplified fragment length polymorphism aflp to the universality ofrandom amplified polymorphic dna rapd.
The fragments were scored as present 1 or absent 0, and those readings were entered in a computer file as a binary matrix one for each marker. The dna fragments produced by rapd and issr markers were shown in figure 3. Screening a higher number of anonymous loci in sugar beet using these molecular markers will enable the selection of the best parent cultivars for the development of novel varieties. Genetic homogeneity revealed using scot, issr and rapd. Population genetic analysis of issr data theresa m. The use of issr and rapd markers for genetic diversity. Issr genotyping of endangered plants using an optimized. Efficiency of using rapd and issr markers in evaluation of. Clustering analysis of ethiopian safflower carthamus. Seven issr primers yielded a total of 252 bands, of. Analysis of population structure of blysmus sinocompressus in. A total of ssr primer sets was used and 84 polymorphic alleles were amplified.
Intersimple sequence repeats issr were used to reveal the genetic diversity and population genetic structure in nine populations of b. Blysmus sinocompressus cyperaceae is an edificator and dominant wetland endemic grass of ecological and economical importance to northwestern china. A comparative analysis of issr and rapd markers for studying genetic diversity in iranian pistachio cultivars ayda tagizad1, jafar ahmadi 2, rahim haddad1 and mahdi zarrabi3 1 agricultural biotechnology department, faculty of engineering and technology, imam khomeini international university, qazvin, iran. A comparative analysis of issr and rapd markers for study.
As an alternative to isozymes, these types of markers are especially attractive given their hypervariable nature, the vast numbers of loci that can be examined, and the small. Inter simple sequence repeat issr polymorphism and its. Genetic diversity among wild and cultivated barley by issr. Issr markers have numerous advantages that make them easy to be applied in several crop studies. A microsatellite is a tract of repetitive dna in which certain dna motifs ranging in length from one to six or more base pairs are repeated, typically 550 times. Shivakumar bakkappa1 and subramanya gopal1 1department of studies in sericulture, university of mysore, manasagangothri, mysore570006, india. Issr is quick, simple, highly reproducible and the use of radioactivity is not essential. Issr dna markers have been widely applied in genetic characterization of desert plant species 2023. Microsatellites occur at thousands of locations within an organisms genome. Issr inter simple sequence repeats as molecular markers to study genetic diversity in tarantulas araneae, mygalomorphae salima machkourmrabet1 4, yann henaut1, ariane dor1, gabriela perezlachaud2, celine pelissier3, charles gers3 and. Molecular markers in plants for analysis of genetic diversity. Singlelocus ssr markers have been developed for a number of species, although there is a major bottleneck in developing ssr markers whereby flanking sequences must be known to design 5anchors for polymerase chain reaction pcr primers. Download the ebook plant biotechnology and molecular markers s. International journal of scientific and research publications, volume 5, issue 3, march 2015 1 issn 22503153.
If the inline pdf is not rendering correctly, you can download the pdf file here. In the present paper, the issr technique was employed to study genetic diversity in italian a. Utility of rapd, issr, irap and remap markers for the. Intersimple sequence repeat issr markers academy of. Twenty eight issr primers amplified a total 244 scorable fragments with average 8. Issr markers resolved two major groups corresponding to the andean and mesoamerican gene pools of common bean. International society for science and religion intersimple sequence repeat, a general term for a genome region between microsatellite loci. High correlation between the rapd and issr marker systems was shown using a mantel test r 0. Inter ssr issr fingerprinting was developed such that no sequence knowledge was required. They have a higher mutation rate than other areas of dna leading to high genetic diversity. Issr inter simple sequence repeats as molecular markers. Srivastava in pdf or epub format and read it directly on your mobile phone, computer or any device.
A testcross population previously used for mapping of rapd and issr markers was used in this study. Osman 18 suggested that utilization of rapd and issr markers could potentially have a high priority for studying genetic diversity and molecular characterization of date palm germplasm. Issr was one of the dnabased markers that have become widely used in various areas of okra genotypes yuan et al. Comparison of rapd, issr, and aflp molecular markers to. Efficiency of ssr, issr and rapd markers in molecular. The cultivars with multiple flower forms, which makes it difficult to make judgment by means of a flowerformbased classification system, have been significantly characterized using leaf morphology or issr markers. The intersimple sequence repeat issr amplification was carried out by a method similar to the previously described by sankar and moore 2001. A total of 120 fragments were detected, with an average of 7. Intersimple sequence repeats issr the generation of issr markers involve pcr amplification of dna using a single primer composed of a microsatellite repeated sequence and in some cases primer also contains 1 4 base anchor at either 3.
Molecular characterisation of cultivars of apple malus. A high level of polymorphism was found with both rapd and issr markers, and the mean polymorphism information content pic values were 0. In this study, we report the use of issr to assess genetic diversity and to determine the relationships among ten cultivars of common bean developed in argentina and three materials from france. This disambiguation page lists articles associated with the title issr.
Nonanchored inter simple sequence repeat issr markers. Assessment of genetic diversity in vigna unguiculata l. In the future, the markers used in this study, in combination with other molecular techniques, could provide a useful panel of issr markers for largescale dna fingerprinting of poplar. Pcr of nuclear dna is a pcr method to map the nuclear genome and to discover rearrangements. The first issr studies were published in 1994 and focused on cultivated species see wolfe and liston, 1998. The comparative analysis of rapd, issr, ipbs and scot markers based on pp, pic, emr and mi values showed the scot markers to be more informative than the other markers. A genomic dna clone representing 1aminocyclopropane1carboxylic acid acc synthase gene, involved in ethylene biosynthesis, was also mapped. Graduate thesis or dissertation genetic diversity of. Species delimitation and genetic diversity analysis in salvia. Because of the multilocus fingerprinting profiles obtained, issr analysis can be applied in studies involving genetic identity, parentage, clone and strain identification, and taxonomic studies of closely related species. Free fulltext pdf articles from hundreds of disciplines, all in one place miscanthus.
The issr primers amplified 91 bands, and 54 showed polymorphism 59. In the present study, intersimple sequence repeat issr markers were employed to investigate the genetic diversity and differentiation of 47 populations sampled from 14 prefectures of the greater mekong subregion. The average polymorphism level with the issr markers 97. Apr 12, 2016 the issr primers amplified 91 bands, and 54 showed polymorphism 59. Inter simple sequence repeat issr pcr is a technique, which involvesthe use of microsatellite sequences as primers in a polymerase chainreaction to generate multilocus markers. Genetic profiling of sugarcane genotypes using inter simple. Intersimple sequence repeat issr markers academy of sciences. The mental test between two jaccards similarity matrices gave r.
Inter simple sequence repeats issr or microsattelite dna. The issr markers make use of microsatellite sequences that are inherently highly variable and ubiquitously distributed across the genome, at the same time achieving higher reproducibility as compared to other genetic markers 18,19. Issr markers for genetic diversity studies in trifolium species. Issr plant genotyping issr genotyping of endangered plants using an optimized work. Research stream flow projection for muar river in malaysia using precis hechms model. Oct 14, 2009 the issr marker is used for the finger printing of cultivars in various crops where less genome information is availble.
Polymorphism detected by issr and rapd markers in issr analysis of triticale genotypes, used issr prim ers, total number n and number of polymorphic bands np, percentage of polymorphic bands %p of issr primers are shown in table 2. It is known for its fine flavour and could represent an important resource for cultivation programs in desert areas. Efficiency of ssr, issr and rapd markers in molecular characterization of mungbean and other vigna species akanksha singh, h k dikshit, neelu jain, d singh and r n yadav1 division of genetics, indian agricultural research institute, new delhi 110 012, india 1 indian. Clustering of isolates remained more or less the same in rapd and combined data of rapd and issr. The aim of this study is the use of rapd and issr markers in assessing genetic diversity in some iraqi. Structure and reticulation analyses revealed some degree of gene fl ow among the species. The white backed planthopper, sogatella furcifera hemiptera. Applicability of leaf morphology and intersimple sequence. Issr markers present a precisely discriminatory power in tree peony cultivar classification without environmental influences.
This research results showed the results show that, firstly, the fertile crescent. The complementary sequences to two neighboring microsatellites are used as pcr primers. An extended linkage map for watermelon based on srap, aflp. Application of inter simple sequence repeat issr markers to. Application of issr markers to fingerprinting of elite.
Inter simple sequence repeat issr markers for assessment of genetic polymorphism and phylogenetic relationships of the silkworm bombyx mori l. Inter simple sequence repeats issr or microsattelite dna this lecture explains the issr which is a type of microsattelite dna in human genome. The linkage map contains 360 dna markers distributed on 19 linkage groups, and covers a genetic distance of 1976 cm with an average distance of 5. Genetic diversity of the planthopper, sogatella furcifera in. The issr is a tool by which programs assess and record the quality of their program and its educational activities in relation to national standards, their institutions mission, the programs goals and objectives, the appropriateness of the curriculum and measured outcomes. Issr polymorphism and its application in mulb erry genome analysis 85 genetic linkage map of issr and rapd markers in ei nkorn wheat in relation to that of r flp markers. Genetic diversity and population structure of haloxylon. Genetic diversity and genotype identification using issr and rapd markers pdf paperity toggle navigation.
Allelic scores, counts and frequencies detected from the accessions of v. Culley dominant markers, such as rapds and issrs, have become popular in molecular studies in recent years. In this study, two microsatellitebased methodologies ssr and issr were evaluated for potential use in fingerprinting and determination of the similarity degree between 41 commercial cultivars of apple previously characterised using rapd and aflp markers. The variation in the performance of the marker systems may be due to the nature and structure of the markers and differences of the genome regions targeted by them talebi et.
Genetic diversity of the planthopper, sogatella furcifera. Efficiency of rapd, issr, ipbs, scot and phytochemical. Presentation mode open print download current view. Analysis of population structure of blysmus sinocompressus. Few molecular studies have been performed on this species. Here we propose, for the first time in spiders, to use issr inter simple sequence repeat technique to study the genetic variability of mexican populations of brachypelma vagans ausserer 1875. Issr markers show differentiation among italian populations. Analysis of genetic diversity among wild and cultivated.
The application of issr markers in genetic variance detection. Issr markers have numerous advantages that make them easy. In the present paper, the issr technique was employed to study genetic diversity. Rapd markers were relatively more efficient than the issr assay. Issr are single primer designed from ssr and amplified region between two. Tan2,3 intersimple sequence repeats issrs are regions in the genome flanked by microsatellite sequences. Authors contributions this work was carried out by the. Pdf the application of issr markers in genetic variance. Indian journal of biotechnology vol, january 2014, pp 8188 efficiency of ssr, issr and rapd markers in molecular characterization of mungbean and other vigna species akanksha singh, h k dikshit, neelu jain, d singh and r n yadav1 division of genetics, indian agricultural research institute, new delhi 110 012, india 1 indian agricultural research institute, regional station, karnal 2 001. The present study showed that issr molecular markers could be used in salvia species delimitation along with morphological study. Species delimitation and genetic diversity analysis in. Inter simple sequence repeat issr markers for assessment of. In addition, issrs are considered useful in gene mapping studies.
Issr usually produces a genomic fingerprint that is similar to that generated by aflp. It is located on a high margin of kavire lut lut desert in the central south of iran and so most of the province is largely steppe or sandy desert and it has hot summers and moderate climate. Characterization of genetic structure using issrpcr markers. These studies demonstrated the hypervariable nature of issr markers.
1219 1312 117 1501 74 38 266 1264 1149 901 296 26 499 1420 1467 555 610 495 1499 489 646 1024 522 1303 1330 879 1497 528 1385 929 1323 26 700 1318 1442 226 678 1210 1171 551 1455 1059